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Pertussis - EAE Induction

Last Updated: Aug 30, 2018 12:41PM PDT
Thank you for you interest in List Labs. 

To follow are a few publications that may aid you with the use of our Pertussis product for EAE induction, please feel free to browse the following:

There are many variations in the methods of induction of EAE, including the strain and gender of
mice, amount of pertussis toxin and the use of adjuvants. Also, we recommend on our Certificate
of Analysis that the optimum dosage be determined for each lot in the particular application.
Below are links to some articles that are free on PubMed; I hope that some of them will be helpful.

1. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4038594/pdf/ppat.1004150.pdf
The inflammasome pyrin contributes to pertussis toxin-induced IL-1β synthesis, neutrophil
intravascular crawling and autoimmune encephalomyelitis. Dumas A, Amiable N, de Rivero Vaccari
JP, Chae JJ, Keane RW, Lacroix S, Vallières L. PLoS Pathog. 2014 May 29;10(5):e1004150. doi:
10.1371/journal.ppat.1004150. eCollection 2014 May. PMID: 24875775

2. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518188/
Connective tissue and its growth factor CTGF distinguish the morphometric and molecular
remodeling of the bladder in a model of neurogenic bladder. Altuntas CZ, Daneshgari F, Izgi K, Bicer
F, Ozer A, Sakalar C, Grimberg KO, Sayin I, Tuohy VK. Am J Physiol Renal Physiol. 2012 Nov
1;303(9):F1363-9. doi: 10.1152/ajprenal.00273.2012. Epub 2012 Sep 19. PMID: 22993071

3. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3533869/pdf/1742-2094-9-245.pdf
Dendritic cell CNS recruitment correlates with disease severity in EAE via CCL2 chemotaxis at the
blood-brain barrier through paracellular transmigration and ERK activation. Sagar D, Lamontagne A,
Foss CA, Khan ZK, Pomper MG, Jain P. J Neuroinflammation. 2012 Oct 26;9:245. doi: 10.1186/1742-
2094-9-245. PMID: 23102113

4. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493354/pdf/2045-8118-9-15.pdf
Active induction of experimental autoimmune encephalomyelitis by MOG35-55 peptide
immunization is associated with differential responses in separate compartments of the choroid
plexus. Murugesan N, Paul D, Lemire Y, Shrestha B, Ge S, Pachter JS. Fluids Barriers CNS. 2012 Aug
7;9(1):15. doi: 10.1186/2045-8118-9-15. PMID: 22870943

5. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347999/
Targeting of prion-infected lymphoid cells to the central nervous system accelerates prion infection.
Friedman-Levi Y, Hoftberger R, Budka H, Mayer-Sonnenfeld T, Abramsky O, Ovadia H, Gabizon R. J
Neuroinflammation. 2012 Mar 21;9:58. doi: 10.1186/1742-2094-9-58. PMID: 22436404

6. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283467/pdf/1742-2094-9-18.pdf
CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during
bacterial toxin exposure and encephalomyelitis. Roy M, Richard JF, Dumas A, Vallières L. J
Neuroinflammation. 2012 Jan 23;9:18. doi: 10.1186/1742-2094-9-18. PMID: 22269426

7. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161869/pdf/1742-2094-8-73.pdf
Lymph node-derived donor encephalitogenic CD4+ T cells in C57BL/6 mice adoptive
transferexperimental autoimmune encephalomyelitis highly express GM-CSF and T-bet. Cravens
PD, Hussain RZ, Zacharias TE, Ben LH, Herndon E, Vinnakota R, Lambracht-Washington D, Nessler S,
Zamvil SS, Eagar TN, Stüve O. J Neuroinflammation. 2011 Jun 24;8:73. doi: 10.1186/1742-2094-8-
73. PMID: 21702922

8. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3121615/pdf/1742-2094-8-49.pdf
Loss of the receptor tyrosine kinase Axl leads to enhanced inflammation in the CNS and delayed
removal of myelin debris during experimental autoimmune encephalomyelitis. Weinger JG, Brosnan
CF, Loudig O, Goldberg MF, Macian F, Arnett HA, Prieto AL, Tsiperson V, Shafit-Zagardo B. J
Neuroinflammation. 2011 May 15;8:49. doi: 10.1186/1742-2094-8-49. PMID: 21569627

9. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012729/pdf/pone.0016009.pdf
Repetitive pertussis toxin promotes development of regulatory T cells and prevents central nervous
system autoimmune disease. Weber MS, Benkhoucha M, Lehmann-Horn K, Hertzenberg D, Sellner
J, Santiago-Raber ML, Chofflon M, Hemmer B, Zamvil SS, Lalive PH. PLoS One. 2010 Dec
30;5(12):e16009. doi: 10.1371/journal.pone.0016009. PMID: 21209857

10. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928301/pdf/pone.0012400.pdf
Centrally administered pertussis toxin inhibits microglia migration to the spinal cord and prevents
dissemination of disease in an EAE mouse model. Yin JX, Tu JL, Lin HJ, Shi FD, Liu RL, Zhao CB, Coons
SW, Kuniyoshi S, Shi J. PLoS One. 2010 Aug 25;5(8):e12400. doi: 10.1371/journal.pone.0012400.
PMID: 20811645

11. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916836/pdf/pone.0011983.pdf
Paradoxical effect of pertussis toxin on the delayed hypersensitivity response to autoantigens in
mice. Yadav R, Bhowmick S, Gorecki P, O'Rourke J, Cone RE.
PLoS One. 2010 Aug 5;5(8):e11983. doi: 10.1371/journal.pone.0011983. PMID: 20700542

12. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2783709/pdf/nihms130483.pdf
Down-modulation of programmed death 1 alters regulatory T cells and promotes experimental
autoimmune encephalomyelitis. Wang C, Li Y, Proctor TM, Vandenbark AA, Offner H. J Neurosci
Res. 2010 Jan;88(1):7-15. doi: 10.1002/jnr.22181. PMID: 19642196

13. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008628/pdf/nihms161118.pdf
A single nucleotide polymorphism in Tyk2 controls susceptibility
to experimental allergicencephalomyelitis. Spach KM, Noubade R, McElvany B, Hickey WF,
Blankenhorn EP, Teuscher C. J Immunol. 2009 Jun 15;182(12):7776-83. doi:
10.4049/jimmunol.0900142. PMID: 19494301

14. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526288/pdf/JPATH173000892.pdf
von-Willebrand factor influences blood brain barrier permeability and brain inflammation
inexperimental allergic encephalomyelitis. Noubade R, del Rio R, McElvany B, Zachary JF, Millward
JM, Wagner DD, Offner H, Blankenhorn EP, Teuscher C. Am J Pathol. 2008 Sep;173(3):892-900. doi:
10.2353/ajpath.2008.080001. Epub 2008 Aug 7. PMID: 18688020

15. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651815/pdf/nihms90391.pdf
IL-13-mediated gender difference in susceptibility to autoimmune encephalomyelitis. Sinha S, Kaler
LJ, Proctor TM, Teuscher C, Vandenbark AA, Offner H.
J Immunol. 2008 Feb 15;180(4):2679-85. PMID: 18250480

16. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1950937/pdf/nihms25316.pdf
Short-term sPECAM-Fc treatment ameliorates EAE while chronic use hastens onset of symptoms.
Reinke EK, Lee J, Zozulya A, Karman J, Muller WA, Sandor M, Fabry Z.
J Neuroimmunol. 2007 May;186(1-2):86-93. Epub 2007 Apr 27. PMID: 17467062

17. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153960/pdf/nihms52472.pdf
Pertussis toxin as an adjuvant suppresses the number and function of CD4+CD25+ T regulatory cells.
Chen X, Winkler-Pickett RT, Carbonetti NH, Ortaldo JR, Oppenheim JJ, Howard OM. Eur J Immunol.
2006 Mar;36(3):671-80. PMID: 16479542

18. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1614706/pdf/JPATH164000883.pdf
Attenuation of Th1 effector cell responses and susceptibility
to experimental allergicencephalomyelitis in histamine H2 receptor knockout mice is due to
dysregulation of cytokine production by antigen-presenting cells. Teuscher C, Poynter ME, Offner H,
Zamora A, Watanabe T, Fillmore PD, Zachary JF, Blankenhorn EP. Am J Pathol. 2004
Mar;164(3):883-92. PMID: 14982842

19. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC182210/pdf/JCI0318648.pdf
A role for surface lymphotoxin in experimental autoimmune encephalomyelitis independent of
LIGHT. Gommerman JL, Giza K, Perper S, Sizing I, Ngam-Ek A, Nickerson-Nutter C, Browning JL. J
Clin Invest. 2003 Sep;112(5):755-67. PMID: 12952924

For more information about our Pertussis products, please visit us at:
https://www.listlabs.com/products/pertussis-toxins-virulence-factors/‚Äč

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