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The ACT (Adenylate Cyclase Toxin) is produced from the cloned cyaA gene of Bordetella pertussis strain 18323, which is expressed as recombinant protein in E. coli K12, in the presence of the acyltransferase CyaC; hence it is fully acylated on residues K860 and K983. The protein sequence is representative of most if not all Bordetella pertussis strains, as there is minimal variation in the sequence of ACT between circulating strains (a single residue difference was identified in the strains sequenced). Only the Adenylate Cyclase domain structure is known; for the rest of the molecule (1322 residues) the structure remains unknown, only the primary sequence is known. Moreover, the protein is stored denatured in 8 M urea, and refolding upon dilution out of urea will depend on handling conditions used.
Attached are two reviews on ACT, both contain useful references that may be of interest to you.
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