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List Labs Product #769 (Anti-Lethal Factor from B. anthracis (Chicken IgY)) can be paired with the List Labs Product #772 (Anti-Lethal Factor from B. anthracis (Goat)), to detect low levels of the Lethal Factor, Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) or Product #169 (Anthrax Lethal Facto (LF-A), Recombinant from B. anthracis Native Sequence).
For example, a 1 µg coating of Product #772 followed by exposure to Lethal Factor and a 1:5,000 dilution of Product #769 gives a midpoint of approximately 100 ng/ml. As low as 5 ng/ml of antigen is readily detected using a standard chromogenic substrate.
In addition, List Labs Product #768 (biotinylated anti-B. anthracis lethal factor, IgY) can be paired with the anti-B. anthracis lethal factor, IgY (List Labs product #769) in a standard sandwich ELISA.
A 1 µg coating of the product #769 antibody is used as the capture antibody followed by exposure to the antigen, Product #169. The antigen is detected using a 1:1000 - 1:2000 dilution of the biotinylated IgY, Product #768 with peroxidase conjugated NeutravidinTM and a fluorogenic substrate. The midpoint of the binding curve is approximately 170 ng/ml. As low as 2.5 ng/ml of antigen is readily detected in the assay.
You may view the Certificate of Analysis (COA) for these products on our website for more details.
For more information about our Anthrax products,
please visit us at: https://www.listlabs.com/products/anthrax-toxins/
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