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The biotinylated anti-C. difficile, Binary Toxin, B Subunit IgY (List Labs product #759) can be paired with the anti-C. difficile, Binary Toxin, B Subunit, IgY (List Labs product #758) in a standard sandwich ELISA.
A 1 µg coating of List Labs product #758 antibody is used as the capture antibody followed by exposure to the antigen (List Labs product #158). The antigen is detected using a 1:1000 - 1:5000 dilution of the biotinylated IgY (List Labs product #759) with a Peroxidase conjugated NeutravidinTM and a fluorogenic substrate. The midpoint of the binding curve is approximately 70 ng/ml. As low as 1 ng/ml of antigen is readily detected.
You may view the Certificate of Analysis (COA) for these products on our website for more details.
For more information about our C. difficile products,
please visit us at: https://www.listlabs.com/products/difficile-toxins/
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